Journal: Journal of Cellular and Molecular Medicine

Article Title: CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH‐NF‐κB signalling pathway

doi: 10.1111/jcmm.14168

Figure Lengend Snippet: Correlations between serum CCL18 levels and clinicopathological parameters in SCCHN patients

Article Snippet: CCL18 levels in the serum of SCCHN patients, precancerous lesions of SCCHN patients and healthy volunteers were determined quantitatively using a human PARC (CCL18) ELISA kit (Raybiotech, Atlanta, GA) according to the manufacturer's protocol.

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH‐NF‐κB signalling pathway

doi: 10.1111/jcmm.14168

Figure Lengend Snippet: Serum level of CCL18 is significantly high in SCCHN patients. A, Levels of CCL18 in the serum of patients with SCCHN, precancerous lesions and healthy controls (* P < 0.05,*** P < 0.001,**** P < 0.0001 vs control). B‐D, ROC analysis showing the area under curve of serum CCL18 to distinguish (B) SCCHN patients from healthy controls, (C) precancerous lesions of SCCHN patients from healthy controls and (D) SCCHN patients from precancerous lesions of SCCHN. CCL18, chemokine (C‐C motif) ligand 18; NC, normal healthy controls; PL, precancerous lesions of SCCHN patients; ROC, receiver operating characteristic; SCCHN, squamous cell carcinoma of the head and neck

Article Snippet: CCL18 levels in the serum of SCCHN patients, precancerous lesions of SCCHN patients and healthy volunteers were determined quantitatively using a human PARC (CCL18) ELISA kit (Raybiotech, Atlanta, GA) according to the manufacturer's protocol.

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH‐NF‐κB signalling pathway

doi: 10.1111/jcmm.14168

Figure Lengend Snippet: CCL18 promotes migration and invasion of SCCHN cells in vitro. Relative expression level of PITPNM3 protein in (A) five different SCCHN cell lines and (B) an immortalized non‐malignant cell line derived from oral mucosa (DOK). C, Expression of PITPNM3 protein in SCCHN cell lines. D‐G, Effect of rhCCL18 on migration (D and F) and invasion (E and G) of SCCHN cells. (* P < 0.05, ** P < 0.01, *** P < 0.001 vs vector). CCL18, chemokine (C‐C motif) ligand 18; DOK, dysplastic oral keratinocyte; rhCCL18, recombinant human CCL18; SCCHN, squamous cell carcinoma of the head and neck

Article Snippet: CCL18 levels in the serum of SCCHN patients, precancerous lesions of SCCHN patients and healthy volunteers were determined quantitatively using a human PARC (CCL18) ELISA kit (Raybiotech, Atlanta, GA) according to the manufacturer's protocol.

Techniques: Migration, In Vitro, Expressing, Derivative Assay, Plasmid Preparation, Recombinant

Journal: Journal of Cellular and Molecular Medicine

Article Title: CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH‐NF‐κB signalling pathway

doi: 10.1111/jcmm.14168

Figure Lengend Snippet: rhCCL18 promotes EMT in SCCHN cells. A, Effect of rhCCL18 on EMT markers at mRNA and (B and C) protein level (** P < 0.01, *** P < 0.001 vs vector). EMT, epithelial‐mesenchymal transition; rhCCL18, recombinant human CCL18; SCCHN, squamous cell carcinoma of the head and neck

Article Snippet: CCL18 levels in the serum of SCCHN patients, precancerous lesions of SCCHN patients and healthy volunteers were determined quantitatively using a human PARC (CCL18) ELISA kit (Raybiotech, Atlanta, GA) according to the manufacturer's protocol.

Techniques: Plasmid Preparation, Recombinant

Journal: Journal of Cellular and Molecular Medicine

Article Title: CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH‐NF‐κB signalling pathway

doi: 10.1111/jcmm.14168

Figure Lengend Snippet: rhCCL18 promotes the expression of MTDH. A, Effect of CCL18 on the expression of MTDH at mRNA and (B and C) protein level (*** P < 0.001 vs vector). CCL18, chemokine (C‐C motif) ligand 18; MTDH, metadherin; rhCCL18, recombinant human CCL18

Article Snippet: CCL18 levels in the serum of SCCHN patients, precancerous lesions of SCCHN patients and healthy volunteers were determined quantitatively using a human PARC (CCL18) ELISA kit (Raybiotech, Atlanta, GA) according to the manufacturer's protocol.

Techniques: Expressing, Plasmid Preparation, Recombinant

Journal: Journal of Cellular and Molecular Medicine

Article Title: CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH‐NF‐κB signalling pathway

doi: 10.1111/jcmm.14168

Figure Lengend Snippet: rhCCL18 promotes migration and invasion of SCCHN cells in an MTDH‐dependent manner. A and C, Migratory changes were examined and quantified by wound healing assay. B and D, Invasive ability was assayed and quantified by Transwell invasion assay. (** P < 0.01, *** P < 0.001) MTDH, metadherin; rhCCL18, recombinant human CCL18; SCCHN, squamous cell carcinoma of the head and neck

Article Snippet: CCL18 levels in the serum of SCCHN patients, precancerous lesions of SCCHN patients and healthy volunteers were determined quantitatively using a human PARC (CCL18) ELISA kit (Raybiotech, Atlanta, GA) according to the manufacturer's protocol.

Techniques: Migration, Wound Healing Assay, Transwell Invasion Assay, Recombinant

Journal: Journal of Cellular and Molecular Medicine

Article Title: CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH‐NF‐κB signalling pathway

doi: 10.1111/jcmm.14168

Figure Lengend Snippet: Knockdown of MTDH impaired the rhCCL18‐induced EMT in SCCHN cells. A, mRNA levels and (B) protein levels. (** P < 0.01) EMT, epithelial‐mesenchymal transition; MTDH, metadherin; rhCCL18, recombinant human CCL18; SCCHN, squamous cell carcinoma of the head and neck

Article Snippet: CCL18 levels in the serum of SCCHN patients, precancerous lesions of SCCHN patients and healthy volunteers were determined quantitatively using a human PARC (CCL18) ELISA kit (Raybiotech, Atlanta, GA) according to the manufacturer's protocol.

Techniques: Recombinant

Journal: Journal of Cellular and Molecular Medicine

Article Title: CCL18 promotes the metastasis of squamous cell carcinoma of the head and neck through MTDH‐NF‐κB signalling pathway

doi: 10.1111/jcmm.14168

Figure Lengend Snippet: NF‐κB activation is essential for CCL18/MTDH‐mediated cell migration, invasion and EMT in SCCHN cells. A, Representative immunofluorescence images show that CCL18 induces nuclear translocation of p65. B, Expression of NF‐κB‐associated protein subsequent to rhCCL18 stimulation. C and D, rhCCL18 enhanced the EMT property featured by E‐cadherin and Vimentin expression (** P < 0.01). This effect of CCL18 is reduced by repressing NF‐κB activation in Tu686 cells. (C) mRNA levels and (D) protein levels. CCL18, chemokine (C‐C motif) ligand 18; EMT, epithelial‐mesenchymal transition; MTDH, metadherin; rhCCL18, recombinant human CCL18; SCCHN, squamous cell carcinoma of the head and neck

Article Snippet: CCL18 levels in the serum of SCCHN patients, precancerous lesions of SCCHN patients and healthy volunteers were determined quantitatively using a human PARC (CCL18) ELISA kit (Raybiotech, Atlanta, GA) according to the manufacturer's protocol.

Techniques: Activation Assay, Migration, Immunofluorescence, Translocation Assay, Expressing, Recombinant

Journal: Journal of Inflammation Research

Article Title: Metformin Modulates Cell Oxidative Stress to Mitigate Corticosteroid-Induced Suppression of Osteogenesis in a 3D Model

doi: 10.2147/JIR.S498888

Figure Lengend Snippet: Impact of different concentrations of prednisolone on macrophage viability and inflammation properties. ( A ) Histogram shows macrophages’ viability on day 7, and ( B ) representative fluorescent image of cells. ( C ) ELISA results of cytokine expressions of IL1b, IL6, TNFα, IL10 and CCL18. (“ns” for “no significant difference”, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: ELISA kits for measuring CCL18 (DY394) and IL1b (DY201-05) were ordered from R&D Systems.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Journal of Inflammation Research

Article Title: Metformin Modulates Cell Oxidative Stress to Mitigate Corticosteroid-Induced Suppression of Osteogenesis in a 3D Model

doi: 10.2147/JIR.S498888

Figure Lengend Snippet: Metformin regulates macrophage functions. ( A and B ) Comparison of ROS level and ATP production in macrophages. ( C ) ELISA results of cytokine expressions of IL1b, IL6, TNFα, IL10 and CCL18. (“ns” for “no significant difference”, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: ELISA kits for measuring CCL18 (DY394) and IL1b (DY201-05) were ordered from R&D Systems.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay

Journal: EBioMedicine

Article Title: Repeated 5-day cycles of low dose aldesleukin in amyotrophic lateral sclerosis (IMODALS): A phase 2a randomised, double-blind, placebo-controlled trial

doi: 10.1016/j.ebiom.2020.102844

Figure Lengend Snippet: Effect of IL-2 treatment on plasma chemokine concentrations. Panels a to c: variation in plasma chemokine levels throughout the study for CCL2 (a), CCL17 (b) and CCL18 (c). Concentrations are expressed as a percentage of baseline value for each individual and points indicate mean values, and error bars their associated SEMs. Open triangles denote individuals receiving placebo, blue squares 1 MIU and black circles 2 MIU of IL2. Three dose comparisons at D64 panels a to c, by the Kruskal-Wallis rank test: (a) p < 0.005; (b) p < 0.0001; (c) p < 0.0028.

Article Snippet: CCL2 and CCL17 plasma levels were measured by Multiplex bead assay (Luminex Human HS Cytokine Panel - R&D Systems), and CCL18 by ELISA (Quantikine ELISA kit (DCL180B, R&D Systems).

Techniques: Clinical Proteomics

Journal: ACS Omega

Article Title: Auxiliary Biomembranes as a Directional Delivery System To Control Biological Events in Cell-Laden Tissue-Engineering Scaffolds

doi: 10.1021/acsomega.6b00502

Figure Lengend Snippet: Release of IL-4 and VEGF from gelatin/HA-tyr films. (A, B) Schematic representation of different loading ways of growth factors inside gelatin/HA-tyr cross-linked films. Release profile determined by the ELISA test over 144 h for VEGF (C, D) and IL-4 (E, F) at 37 °C in PBS. For C–F, fresh supernatant was added after each record and three samples for each condition were used.

Article Snippet: The levels of cytokines were measured with ELISA Development Kits (VEGF, IL-4, IL1-RA, IL-6, IL-8, TNF-α, CCL18, PeproTech).

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Disease Markers

Article Title: Comparative Study of Circulating MMP-7, CCL18, KL-6, SP-A, and SP-D as Disease Markers of Idiopathic Pulmonary Fibrosis

doi: 10.1155/2016/4759040

Figure Lengend Snippet: Serum levels of (a) MMP-7, (b) CCL18, (c) KL-6, (d) SP-A, and (e) SP-D in patients with IPF, those with bacterial pneumonia (BP), and healthy controls (HC). The significant differences between the three groups were evaluated using Bonferroni's test ( ∗ p < 0.01 and ∗∗ p < 0.001).

Article Snippet: MMP-7, CCL18, SP-A, and SP-D were measured by commercially available enzyme-linked immunosorbent assay (ELISA) kits (Human Total MMP-7 Quantikine ELISA Kit, R&D Systems, MN; Human CCL18/PARC Quantikine ELISA Kit, R&D Systems, MN; SP-A Test Kokusai-F Kit, Sysmex, Japan; and SP-D EIA Kit Yamasa, Yamasa, Japan).

Techniques:

Journal: Disease Markers

Article Title: Comparative Study of Circulating MMP-7, CCL18, KL-6, SP-A, and SP-D as Disease Markers of Idiopathic Pulmonary Fibrosis

doi: 10.1155/2016/4759040

Figure Lengend Snippet: Cut-off values and the discriminatory ability of five biomarkers by ROC curve analysis, which distinguishes IPF patients ( n = 65) from control subjects ∗ ( n = 132).

Article Snippet: MMP-7, CCL18, SP-A, and SP-D were measured by commercially available enzyme-linked immunosorbent assay (ELISA) kits (Human Total MMP-7 Quantikine ELISA Kit, R&D Systems, MN; Human CCL18/PARC Quantikine ELISA Kit, R&D Systems, MN; SP-A Test Kokusai-F Kit, Sysmex, Japan; and SP-D EIA Kit Yamasa, Yamasa, Japan).

Techniques: Control, Diagnostic Assay

Journal: Disease Markers

Article Title: Comparative Study of Circulating MMP-7, CCL18, KL-6, SP-A, and SP-D as Disease Markers of Idiopathic Pulmonary Fibrosis

doi: 10.1155/2016/4759040

Figure Lengend Snippet: Cox proportional hazards model to predict the 5-year mortality of patients with IPF.

Article Snippet: MMP-7, CCL18, SP-A, and SP-D were measured by commercially available enzyme-linked immunosorbent assay (ELISA) kits (Human Total MMP-7 Quantikine ELISA Kit, R&D Systems, MN; Human CCL18/PARC Quantikine ELISA Kit, R&D Systems, MN; SP-A Test Kokusai-F Kit, Sysmex, Japan; and SP-D EIA Kit Yamasa, Yamasa, Japan).

Techniques:

Journal: Disease Markers

Article Title: Comparative Study of Circulating MMP-7, CCL18, KL-6, SP-A, and SP-D as Disease Markers of Idiopathic Pulmonary Fibrosis

doi: 10.1155/2016/4759040

Figure Lengend Snippet: Kaplan-Meier analysis to evaluate the probability of 5-year survival among the three groups which were divided according to the serum levels of KL-6 and MMP-7. The cut-off levels of KL-6 and MMP-7 were 1040 U/mL and 9.67 ng/mL, respectively. The probability of 5-year survival was significantly different among them ( p = 0.0004).

Article Snippet: MMP-7, CCL18, SP-A, and SP-D were measured by commercially available enzyme-linked immunosorbent assay (ELISA) kits (Human Total MMP-7 Quantikine ELISA Kit, R&D Systems, MN; Human CCL18/PARC Quantikine ELISA Kit, R&D Systems, MN; SP-A Test Kokusai-F Kit, Sysmex, Japan; and SP-D EIA Kit Yamasa, Yamasa, Japan).

Techniques: